ELISA Plates for ELISA Assay Kit

Add control: add 3 wells of negative control, 1 well of positive control, and 100ul of control serum.
Incubate: The reaction ELISA 96 Well Plate needs to be shaken to make the sample fully mixed, and then placed in a 37°C incubator or water bath for 20 minutes of reaction.
Wash the ELISA Plate: Dilute the washing solution 15m with distilled water to a volume of 300ml. When hand washing, the contents in the wells of the reaction plate should be poured out, the washing solution should be filled to the position of the reaction wells, and then placed for 30 minutes and then shaken off vigorously. Repeat this action for 5 times and then start to pat dry. Machine washing needs to be carried out 5 times, each hole is injected with 200u of washing solution or can be filled, and then stop
After 30 minutes, drain and pat dry.
Add enzyme labeling working solution: Add 100ul of enzyme labeling working solution to each well, place it in an incubator at 37, and wash the ELISA Microplate 5 times after 20 minutes of reaction. The operation of washing the ELISA Microplate is the same as the above steps.
This is the first step of the reaction, which is color development and termination of the reaction: 50ul of the substrate is added to the reaction well, and the color is developed for 10 minutes in the dark under 37 conditions. Add stop solution to each well
50ul was mixed to stop the reaction.
The use of Bunsen microtiter plates needs to be carried out under sterile conditions. Disinfection and cleaning should be done carefully after using the microtiter plate, but it should be noted that some enzymes
Before disinfection of the standard board, you need to read the instructions carefully to see if it can be sterilized at high temperature.