Mbale Yakuya Kwambiri

tili ndi mitundu yambiri ya 96 Well Deep Well Plate, monga 0.5ml elution plate, 1.2ml square well V pansi mbale, 2.0ml round well U botttom plate kwa Hamilton ndi Nunc machine, 2.2ml square well 96 Deep Well Plate V Pansi ndi 2.2ml lalikulu bwino U pansi. Iwo akhoza chosawilitsidwa pansi atolankhani mkulu, ndi kusunga mawonekedwe kwa mphindi 20 ngakhale kutentha kufika pa 121 ℃, makhalidwe onsewa anapereka muyezo watsopano kwa labotale.

Ikhoza kusungidwa mufiriji mpaka -80 madigiri;
Ingathe kupirira mphamvu yaikulu ya centrifugal ya 3000-4000g popanda kuwonongeka kapena kupindika;
Akhoza autoclaved, 121 ℃, 20psi, palibe mapindikidwe kwa mphindi 20, ngakhale Kutentha;
Pang'ono yotsalira madzi, zochepa khoma atapachikidwa chodabwitsa; zitsulo zotsika kwambiri;
Kugwiritsa ntchito pulasitiki yochokera kunja kuyeretsedwa kwa polypropylene (PP) zopangira, zotsutsana kwambiri ndi dzimbiri za mankhwala, monga DMSO, phenols, chloroform; inert kwa madzi;
Pambuyo poyendera gulu lachitatu, palibe kuipitsidwa kwa enzyme ya RNA / DNA;
Itha kugwiritsidwa ntchito pamakina odzichitira okha, ndipo kukula kwake kumagwirizana ndi muyezo wa SBS;
Itha kugwiritsidwa ntchito ndi zomata, gel osakaniza, osindikizidwa, osatulutsa;
Kusalala kwabwino, ndipo kumatha kunyamulidwa mtunda wautali pambuyo posindikizidwa ndi filimu yosindikizira kutentha;
Zogulitsazo zimapangidwa ndikuyikidwa mumsonkhano wopanda fumbi wa 100,000, ndipo mtundu wazinthuzo ndi wokhazikika.