DNASE RNASE Free 96 PCR PLAN

Ma module a Yongoe 156-Chabwino ndi ma module 384 amapangidwa mogwirizana ndi miyezo yapamwamba kwambiri. Zotsatira zoyesedwa ndi yunifolomu, ndipo khoma la chubu limakhala lochepa thupi komanso yunifolomu, lomwe limatsimikizira kuti kutentha kwa kutentha ndi kuwotcha yunifolomu. 8 PCR Tuba states / 12 pcr mapira amatha kugwiritsidwa ntchito. Kuphimba kapena filimu yosindikiza. Tili ndi mitengo ya 96 ya PCR ndi 384 PCR PLUNTS.

1. Kupanga kwa polypropylene (ma pp) zida;

2. Oyenera 0.1ml ndi ma module 0.2ml kutentha kwa marcle;

3. Wopanda dnase, rnase ndi endotoxin;

"Monga tonse tikudziwa, PCR ndi njira yoyeserera yoyesera mu laboories yazogwira ntchito." Zotsatira zoyeserera nthawi zonse sizikhala zosakhutiritsa, zomwe zitha kuwonongeka pang'ono za PCR pulasitiki zomata, kapena kuyambitsa zoletsa zosokoneza. Pali chifukwa china chofunikira kwambiri: kusankha kolakwika kwa zotayikira kumathandizanso kwambiri pazotsatira.

Pali zifukwa zambiri zomwe zimakhudza zotsatira za zoyeserera za PCR: nthawi zambiri pamakhala mitundu 7 yotsatirayi.

1. Primers: Primers ndi fungulo la zomwe PCR ikuchitika. Zojambula za PCR zimatengera kuchuluka kwa kumaliza pakati pa primers ndi template DNA;

2. Enzyme ndi ndende yake;

3. Mtengo ndi ndende ya Dntp;

4. Template (gener Gene) nuclec acid;

5. Mg2 + kukwiya;

6. Kutentha ndi nthawi ikukhala;

7. Nthawi yozungulira; 8. Zida, zosemphana, ndi zina zambiri.

Zina mwazinthu zomwe zimapangitsa zinthu zambiri, zosemphana ndi imodzi mwazinthu zofunika kwambiri komanso zosayembekezereka. Zovala za PCR zikupezeka m'mitundu yosiyanasiyana: 8-tubulu pcr chubu, machubu otsika mtengo, ma pign a pcr ndi qpcr mbale za pcr ndi qpcr mokweza. Chisankho ndi chovuta kwambiri ndipo pali zovuta zambiri.

Wonyadiraci Medical ndi wopanga limodzi. QPCR imafunikira kuzindikira kwamphamvu kwa fluorescescent, ndipo koposa zonse, chovuta komanso cholondola. Poyerekeza ndi zinthu za PCR yowonekera ya PCR, zopangidwa zoyera zimatha kuwonetsa zizindikiro za Fluorescent, kuchepetsa kuipitsidwa ndi zizindikiro pakati pa zitsime, ndikutha kungotsitsimutsanso ma fluorescent yeniyeni pcr.

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