Laupapa Vailoloa

e tele a matou ituaiga 96 Well Deep Well Plate, e pei o le 0.5ml elution plate, 1.2ml square well V plate bottom, 2.0ml round well U bottom plate mo Hamilton ma Nunc machines, 2.2ml square well 96 Deep Well Plate V Lalo ma 2.2ml sikuea lelei U pito i lalo. E mafai ona faʻamaʻiina i lalo o le lomitusi maualuga, ma tausia le foliga mo le 20 minute e oʻo lava i le vevela e oʻo i le 121 ℃, O nei uiga uma e faʻatulaga ai se tulaga fou mo le fale suesue.

E mafai ona fa'aisa ma fa'a'aisa e o'o atu i le -80 tikeri;
E mafai ona tatalia le malosi maualuga centrifugal o 3000-4000g e aunoa ma le faaleagaina po o deformation;
E mafai ona autoclaved, 121℃, 20psi, leai se deformation mo 20 minute, e oo lava i le vevela;
Fa'aitiitia le vai totoe, fa'aitiitia le fa'ailoga puipui; maualalo mea mamafa mamafa;
Fa'aaogāina fa'aulufale mai palasitika maualuga mama mama umi polypropylene (PP) fa'ameamata, fa'atasi ai ma le lelei tele o le tete'e atu i vaila'au fa'ama'i, e pei o le DMSO, phenols, chloroform; lega i le vai;
A maeʻa suʻesuʻega a le isi vaega, e leai se faʻaleagaina o le enzyme RNA / DNA;
E mafai ona faʻaaogaina i masini faʻapitoa, ma o le tele o oloa e fetaui ma le tulaga SBS;
E mafai ona faʻaaogaina i mea faʻapipiʻi, silica gel, faʻamaufaʻailogaina, leai se tafe;
E lelei le mafolafola, ma e mafai ona feaveaʻi mo se mamao umi pe a uma ona faʻamaufaʻailogaina i le vevela-faʻamauga ata;
O oloa e gaosia ma faʻapipiʻiina i totonu o se faleaoga 100,000-vasega e leai se pefu, ma e mautu le lelei o oloa.