I-DNAK RANEA Free I-96 kakuhle iplate ye-PCR

I-YongYyee yezonyango i-96 yemilinganiselo yemodyuli kunye neemodyuli ezingama-384 ziveliswa ngokungqongqo ngokungqinelana neyona migangatho iphezulu. Iziphumo zovavanyo ziyinto iyunifomu, kwaye udonga lwe-tube luphambile kwaye iyunifomu, eqinisekisa ubungakanani bokuhambisa ubushushu kunye nokufudumeza kwesampulu. I-8 pcr tubes / 12 i-pubes ingenziwa. Gquma okanye ukutywina ifilimu. Sine-96 kakuhle ipleyiti ye-PCR kunye ne-384 kakuhle ipleyiti ye-PCR.

1. Ukuveliswa kweZixhobo zePolypropylene (PP);

2. Ifanelekile kwi-0.1ML kunye neemodyuli zemodyuli ze-thermal yomjikelo;

3. simahla kwi-dnase, rnase kunye ne-endotoxin;

"Njengoko sonke sazi, i-PCR yindlela esisiseko yeelebhu ze-biochemical." Iziphumo zovavanyo zihlala zingonelisi, ezinokubangelwa kukungcoliseka okuncinci kwezinto ezisetyenziswayo zeplastiki, okanye ukumiliselwa kwezithintelo ezikhokelela ekuphazamiseni kovavanyo. Kukho esinye isizathu esibalulekileyo: Ukukhetha okungalunganga kwezinto ezinokuthethwa yimpembelelo enkulu kwiziphumo zovavanyo.

Zininzi izizathu ezichaphazela iziphumo zovavanyo lwe-PCR lovavanyo: Ziindlela ezihlala zineentlobo ezisi-7 zilandelayo.

1. I-Primers: I-Primers yeyona nto iphambili kwi-PCR Reage. Ubuso beemveliso ze-PCR zixhomekeke kwinqanaba lokucekisa phakathi kwe-primers kunye netemplate ye-template;

2. i-enzyme kunye nengcinezelo yayo;

3. Umgangatho kunye nokugxininiswa kwe-DNTP;

4. Itemplate (ekujoliswe kuyo i-gene) ye-nucleid;

5. I-MG2 + ixilongo;

6. Ubushushu kunye nokubekwa kwexesha;

7. Ixesha lomjikelo; 8. Izixhobo, izinto ezinokusetyenziswa, njl.

Phakathi kwezinto ezininzi zokuphembelela, izinto ezinokusetyenziswa zenye yezona zinto zibalulekileyo nezingaphezulu. Izitrato ze-PCR ziyafumaneka ngeendlela ezahlukeneyo: I-8-tube ye-tube ye-PCR, iityhubhu eziphantsi, iityhubhu ezisemgangathweni, i-STRAT ye-PCR ye-PCR kunye ne-SCRCR Plates ezinokuphakanyiswa. Ukukhetha kunzima kakhulu kwaye zininzi iingxaki ezininzi.

I-Younie yezonyango ngumvelisi wokumiswa komntu. I-QPRCR ifuna ukubongwa kwexabiso le-Fluorescent uphawu lwe-Fluorescent, kwaye okona kubaluleke ngakumbi, i-fluorescent isibonisi. Xa kuthelekiswa neemveliso ze-PCR ezibonakalayo zemveli, iimveliso ezimhlophe ze-PCR ezimhlophe zibonisa imiqondiso ye-fluorescent, ukunciphisa ungcoliseko lwemiqondiso phakathi kwamaqula, kwaye uqiniseke i-PluoSCT iziphumo ze-PCR.

Iinkcukacha ezithe kratya

Iinkcukacha ezithe kratya