I-DNASE RNASE MAHHALA 96 Well Plate

Amamojula ajwayelekile we-Yongyue Medical 96-Well kanye namamojula angama-384-kahle akhiqizwa ngokuhambisana namazinga aphezulu kakhulu. Imiphumela yokuhlola iyunifomu, futhi udonga lwe-tube mncane futhi umfaniswano, oluqinisekisa izinga lokudlulisa ukushisa kanye nokushisa okufanayo kwesampula. Amashubhu we-8 PCR / 12 PCR TABses angasetshenziswa. Ikhava noma ifilimu yokubopha uphawu. Sine-96 kahle PCR PLUATES kanye namapuleti angama-384 Well PCR.

1. Ukukhiqizwa kwezinto zokwenziwa zePolypropylene (PP);

2. Ilungele i-0.1ML ne-0.2ml Thermal Cycle Module;

3. Mahhala kwe-DNASE, i-Rnase ne-Endotoxin;

"Njengoba sonke sazi, i-PCR iyindlela eyisisekelo yokuhlola ezindaweni zokuphumula ze-biochemical." Imiphumela yokuhlola ihlala ingagculisi, okungenzeka ngenxa yokungcoliswa okuncane kwePcr Plastic Cook, noma ukwethulwa kwama-inhibitors okuholela ekuphazanyisweni kokuhlolwa. Kunesinye futhi isizathu esibaluleke kakhulu: Ukukhetha okungalungile kokudla nakho kuba nomthelela omkhulu emiphumeleni yokuhlola.

Kunezizathu eziningi ezithinta imiphumela yokuhlolwa kwe-PCR: kuvame ukuba zinhlobo ezilandelayo eziyi-7.

1. Ama-Primers: ama-primers ayisihluthulelo sokuphendula okuthile kwe-PCR. Ukucaciswa kwemikhiqizo ye-PCR kuncike ngezinga lokuhambisana phakathi kwama-primers ne-template DNA;

2. I-enzyme nokuhlushwa kwayo;

3. Ikhwalithi kanye nokuhlushwa kwe-DNTP;

4. ithempulethi (i-getge gene) nucleic acid;

5. I-MG2 + ukugxila;

6. Ukuhlelwa kokushisa nesikhathi;

7. Isikhathi sokujikeleza; 8. Emishini, okudliwayo, njll.

Phakathi kwezinto eziningi ezinethonya, izifebe zingenye yezinto ezibaluleke kakhulu futhi ezinakwa kalula. Ukudla kwe-PRR kuyatholakala ezinhlotsheni ezahlukahlukene: Imichilo ehlukahlukene ye-8 Ukukhetha kunzima kakhulu futhi kunezinkinga eziningi ezivamile.

I-Yonnwee Medical ingumkhiqizi wokumisa okukodwa. I-QPCR idinga ukutholwa kwenani lesikhathi sangempela sokuqina kwesiginali ye-fluorescent, futhi okubaluleke kakhulu, okubucayi futhi okunembile ukuhanjiswa kwesiginali we-fluorescent. Uma kuqhathaniswa nemikhiqizo yendabuko ye-PCR ye-PCR, imikhiqizo emhlophe ye-PCR ingabonisa kakhulu amasiginali we-fluoreporescent, inciphise ukungcoliswa kwezimpawu zokulinganisa phakathi kwemiphumela ye-PCR ye-PCR yesikhathi sangempela.

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