In PCR experiments, there are many factors that affect the amplification effect. We understand the quality and concentration of dNTPs, the amount and degree of purification of template nucleic acid, the design of primers, the concentration of DNA polymerase, and the length of amplification. In fact, the consumables used may also affect the results of the experiment, which is often overlooked by us. Therefore, before choosing experimental consumables, we also need to understand the characteristics and terminology related to PCR consumables. According to the different detection methods, PCR experiments are divided into ordinary PCR methods and fluorescent quantitative PCR methods. The ordinary PCR method is generally the end-point PCR running gel method, so the thermal conductivity and sealing performance of the consumables used are inseparably related to the experimental results; while the fluorescent quantitative PCR is the real-time detection of fluorescent signals, in addition to thermal conductivity and sealing performance, it is Light and light resistance are also required. In short, the consumables for fluorescent quantitative PCR can also be used for ordinary PCR, but not vice versa. The selection of fluorescent quantitative PCR consumables needs to pay attention to the following four aspects: 1. Tightness The better the sealing, the less the product volatilizes, the more products are obtained, and the less aerosols are produced, and the less pollution for the next round of experiments, which means the lower the rate of false positives. . 2. Thermal conductivity The better the thermal conductivity and the more uniformity, the faster the PCR speed and the higher the accuracy. Three, light transmittance Mainly for fluorescence quantification, we know that it is a real-time quantification method, so the light transmittance of the consumable itself is directly related to the amount of fluorescence signal we detect. If you choose a consumable that has good light transmittance, the signal that needs to be detected will become weak, which will cause us to misjudge the result. Four, protection from light This is for fluorescence quantification. If we do not pay attention to avoiding light during the entire experiment, and the fluorescent reagents are excessively consumed, it will directly affect the amplification efficiency of our experiment and lead to inaccurate results. Depending on the detection method, the choice of consumables is also different. Therefore, it is necessary to select the corresponding consumables according to the PCR experiment. PCR/qPCR consumables are generally made of polypropylene (PP). Most of the volume specifications of PCR Tubes can meet the requirements of PCR reactions. Yongyue Medical PCR tubes are available in different colors and specifications, including transparent and white colors. , The tube body specifications are available in 1ml and 2ml, and there are two types of PCR tubes and 8 Strip PCR Tubes, which can meet the different experimental needs of customers.
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