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June 20, 2023

How to clean the cell culture dish when using it

Cell Culture Dish (150mm): The special structural improvement design increases the strength of the lid and the bottom of the dish, improves the flatness at the same time, and increases the adsorption of the inner wall of the Culture Dish.

Cleaning steps for Cell Culture dishes:

Generally, it goes through four steps of soaking, scrubbing, pickling, and cleaning.


cell culture dish


1. Soaking: New or used glassware should be soaked in water first to soften and dissolve the attachments. New glassware should be simply scrubbed with tap water before use, and then soaked overnight in 5% hydrochloric acid; used glassware often has a lot of protein and oil attached to it, which is not easy to wash off after drying, so it should be immersed in clean water immediately after use for scrubbing .

2. Scrubbing: Put the soaked glassware in detergent water and scrub it repeatedly with a soft brush. Do not leave dead space and prevent damage to the surface finish of the utensils. Wash and dry the cleaned glassware for pickling.

3. Pickling: Pickling is to soak the above-mentioned utensils in a cleaning solution, also known as acid solution, to remove possible residual substances on the surface of the utensils through the strong oxidation of the acid solution. Pickling should not be less than six hours, usually overnight or longer. Be careful with the utensils.

4. Rinse: The utensils after scrubbing and pickling must be fully rinsed with water. Whether the utensils are cleaned after pickling will directly affect the success or failure of cell culture. Hand-wash the utensils after pickling, and each utensil must repeat "water filling-emptying" at least 15 times, and finally soak it with double-distilled water for 2-3 times, dry or dry it and pack it for later use.
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