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Some time ago China's epidemic is occurring at multiple points and local outbreaks Some areas have successively launched Nucleic acid testing for all employees Nucleic acid testing is a "monitoring network" for epidemic prevention and control On August 10 China Health Commission issued
Scientific research dry goods | 10 common strategies for PCR
The PCR experiment includes three main thermal cycling steps. In this process, a variety of necessary reaction components are required. By adjusting the PCR settings accordingly, some special experimental results can be obtained, such as increased yield and enhanced specificity. Or shorten the reaction time. Hot start PCR It is often used to enhance the specificity of PCR amplification. This method mainly uses enzyme-modified enzymes such as antibodies, affinity ligands
How to perform a new coronavirus nucleic acid test? Take you to reveal the whole process
Since the occurrence of COVID-19, the relatively rare medical term "nucleic acid testing" has become a high-frequency vocabulary in our lives. The nucleic acid test that most people see is just the process of sampling by medical staff. Where will the specimen go later? What are the "fully armed" inspectors doing in that closed laboratory? Today, Yongyue Medical takes you to the laboratory to find out and understand the whole process of the new coronavirus nucleic acid detection.
PCR Lab: How to prevent false positives in COVID-19 nucleic acid testing?
Nucleic acid testing is the "gold standard" for COVID-19 testing. Real-time fluorescent quantitative RT-PCR is a common method for clinical testing. With the widespread use of this method in laboratories at all levels, false positives in clinical testing of large quantities of samples cannot be ignored. The problem. In simple terms, the false positive of real-time quantitative PCR means that a fluorescent signal appears in a sample that should not have a fluorescent signal, which leads to a misjudgment of a positive result.
I believe that everyone is no stranger to COVID-19. Its emergence in 2019 can be said to have turned the world upside down. Until now, it is still doing evil all over the world. In China, under the correct leadership of the Party Central Committee and the joint efforts of the people across the country, we are united to fight against COVID-19. Extinguished the ambition
Speaking of the COVID-19 epidemic, everyone may feel it. After all, until today, its impact still covers all parts of the world. Not only that, if there is a slight negligence, it may cause a new round of epidemics, and Southeast Asia has obviously been "successful" recently. We know that before June, the epidemic situation in Southeast Asian countries such as Vietnam and Myanmar was still stable, but now the epidemic situation in these countries has taken a turn for the worse, and they have begun to struggle in a new round of the epidemic.
Meet the "Delta Strain"! How is it different from "COVID-19"?
Recently, the exposure of the "Delta" strain has surged! "Delta" virus, also called delta virus, is one of the mutated strains of the new crown. It was first discovered in India on October 5, 2020 and has become the main epidemic strain of the global epidemic. What are the characteristics of the Delta strain?
Real-time fluorescence quantitative PCR experiment operation process
1. Experimental equipment and reagents 1. Experimental equipment Multi-sample grinding bead homogenizer Desktop high-speed refrigerated microcentrifuge Fluorescence quantitative PCR instrument Clean bench Spectrophotometer Centrifuge tube
What are the characteristics of the Delta strain? First, the communication capability has been significantly enhanced. According to a study by the World Health Organization, the transmission rate of Delta virus has increased by nearly 100% compared with other non-VOC and older virus strains, which is doubled. In the recent outbreak of the new crown pneumonia caused by the delta mutant virus, research on the transmission dynamics also suggests that the infectivity has also doubled compared with the previous epidem
3 million medical supplies rush to Xinxiang
On July 25, the transferred people took a rest in the dormitory of the second middle school resettlement site in Xinxiang City, Henan Province. A reporter from Xinhua News Agency learned from the Flood Control and Drought Relief Headquarters of Xinxiang City, Henan Province on the 26th that as of 11 o'clock on the 25th, heavy rains had caused disasters
Each step of a complete pipetting cycle of the pipette must follow the operating specifications
The working principle of the pipette is that the piston achieves liquid suction and discharge through the telescopic movement of the spring. Under the push of the piston, part of the air is discharged, the liquid is sucked in by the atmospheric pressure, and then the air is pushed out by the piston to discharge the liquid. When in use, it can be operated in accordance with the elasticity of the spring, which can well control the speed and strength of pipetting. A complete pipetting cycle of the pipette, includ
Five inspirations left to China from the heavy rain disaster in Henan!
The heavy rain is still falling, and the alarm has not been lifted. The hearts of all Chinese people are tugging for Henan and Zhengzhou. The tr
Types and characteristics of 96 deep-well plates
classification: 1. According to the number of wells, the more common ones can be divided into 96-well plate and 384-well plate. 2. According to the hole type, 96 deep-well plate can be divided into round hole type and square hole type. The 384-well plates are all square-well type. 3. According to the shape of the bottom of the hole, there are mainly two types: U-shaped and V-shaped.
Regarding the selection of pipette tips, only the insider knows the secret
A pipette can only be used with a good tip! The tip is the most important factor affecting accurate pipetting. The pipette and the tip together form a complete pipetting system. • Low suction tip: • Low adsorption pipett
Does the cryotube need to be sterile?
The cryopreservation of cells using cryopreservation tubes is one of the main methods of cell preservation. Using cryopreservation technology to store the cells in liquid nitrogen at -196°C at low temperatures can temporarily leave the cells out of the growth state and preserve their cell characteristics, so that the cells can be resuscitated for experiments when needed. In addition, proper preservation of a certain amount of cells can prev
How to select materials for laboratory centrifuge tubes
Centrifuge tube is a common experimental consumable in the laboratory, and it is mainly used in conjunction with the centrifugal dehydrator. The liquid to be tested is packed in this, and then placed in the centrifugal dehydrator for suction filtration. According to the different materials
First of all, everyone knows that there are many types of laboratory consumables. For example, biological and chemical laboratories commonly use chemical reagents, biological reagents, reference materials, reference materials, consumables (test paper/filter paper/glassware, etc.). The procurement, storage, management and use of reagents and consumables are important tasks of the laboratory. , And the most common tasks are outgoing, warehousing, purchasing, and requisition.
●Designate the area where the PCR system is prepared to minimize the entry and exit of items. ●Wear gloves when working in this area and change them frequently. Wear a mask and headgear to reduce the pollution of facial skin and hair cell sources. ●Establish a set of reagents and disposable supplies (including PCR tubes, mineral oil, wax beads, etc.) for individual use. Use new glassware, plastic utensils, and pipettes that have not been exposed to the DNA
How to use pipettes and precautions!
A complete pipetting cycle includes the steps of tip installation-volume setting-aspiration and dispensing-removal of the tip. Each step has operating specifications that need to be followed. 1. Suction head installation For single-channel pipettes, insert the pipette tip vertically into the pipette tip, press lightly and t
Dry goods | Inhibitors in a sample that affect PCR detection
To successfully convert a sample into a PCR result, there are many steps that need to be paid attention to in the early stage, including: sample collection, transportation, storage, and nucleic acid extraction. Each step needs to be careful, and the middle process can be described as difficult. In the process of converting samples into PCR results, what factors make the conversion process so difficult?
Reasons and solutions for pipette failure
Tips of several common liquid withdrawal failures: 1. There is residual liquid in the pipette nozzle: This situation occurs because the suction nozzle is not suitable, and the original suction nozzle of the pipette should be replaced immediately. 2. The pipette is blocked and the suction volume is too small: This happens because the liquid has penetrated into
What is a tip? What is a good tip? How to choose the tip?
Classification of pipette tips Tips, as consumables used with pipettes, generally can be divided into: ①. Standard tips, ②. Filter tips, ③. Low adsorption tips, ④. No heat source, etc. 1. Standard tip is the most widely used tip. Almost all pipe
Pipettes achieve different accuracy and precision through a variety of designs, including simple pipettes made of a piece of glass, to complex adjustable or electronically controlled pipettes. The accuracy of measurement varies greatly depending on the type . Today, Sister Xiaoxi will have a good chat with everyone about pipettes. Pipettes, also known as "quantitative pipettes", "pipettes", and "pipettes" are experimental instruments designed to measure the volume of liquids and transfer them to other containers.
What is a pipette tip and the function of a filter tip?
Pipette tips Yongyue Medical supplies: pet indwelling needles, fluorescent quantitative PCR consumables, pipette nozzles, microcentrifuge tubes, imported cryopreservation tubes, cell culture dishes, culture plates, culture bottles, imported tips, instruments and Gloves, chromatography consumables, syringe filters, etc. The pipette is an experimental instrument in biological research, and the number of its accessory tips in the experiment is also very large. The tips on the market are basically made of polyprop
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Privacy statement: Your privacy is very important to Us. Our company promises not to disclose your personal information to any external company with out your explicit permission.