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Daily management of laboratory cell consumables
As an important carrier for major scientific research institutions, universities, and pharmaceutical companies to carry out biological cell research, the laboratory is of great significance in promoting the development of biomedicine and ensuring the smooth progress of teaching. However, the management of cell consumables is often overlooked during the use of laboratories, which is not conducive to the smooth progress of research work to a certain extent. Therefore, it is particularly important to strengthen the daily management of laboratory cell consumable
"Who" can help you easily detect cell viability
Cell Counting Kit is abbreviated as CCK-8 kit, which is based on WST-8 (chemical name: 2-(2-methoxy-4-nitrobenzene)-3-(4-nitrophenyl)-5-( 2,4-Disulfobenzene)-2H-tetrazole monosodium salt) is a rapid and highly sensitive detection kit widely used in cell proliferation and cytotoxicity. WST-8 can be reduced by dehydrogenase in mitochondria to a highly water-soluble yellow formazan product in the presence of electronic coupling reagents. The intensity of the color is directly proportional to cell proliferation and inversely proportional to cytotoxicity. Use a microplate reader to measure th
What is the difference between ordinary PCR technology and digital PCR technology?
Since the advent of PCR technology, it has played a huge role in the field of molecular diagnosis of genetic diseases, pathogens, oncogenes, and forensic identification. According to the evolution of PCR technology, people habitually divide PCR technology into three generations: ordinary PCR technology, real-time fluorescent quantitative PCR technology (qPCR) and digital PCR (dPCR) technology. I have also introduced a lot of knowledge about qPCR in the past. In this issue, I will share the other two PCR technologies-ordinary PCR technology and dPCR technolog
The experiment tells you! Which pipette or pipette is more accurate!
Which pipette or PK pipette is more accurate? In this article, we will discuss the structure of pipettes and pipettes and the effect of liquid aspiration principles on the accuracy of actual use with examples. I believe that after reading this article, my friends will have a deeper understanding of the accuracy comparison between pipettes and pipettes. The same calibration method Before that, we must first know that the method of calibration (calibration) of pip
Experience in purchasing consumables such as pipettes and tips
A pipette can only be used with a good tip! The tip is the most important factor affecting accurate pipetting. The pipette and the tip together form a complete pipetting system. • Low suction tip: • Low adsorption pipette tips Compared with ordinary pipette tips, the adsorption of samples is reduced by 3-5 times
PCR plate is a kind of polymerase chain reaction (Polymerase Chain Reaction, PCR), which is mainly used as a carrier for primers, Taq DNA polymerase, dNTP, template nucleic acid, Mg, buffer, etc. involved in the amplification reaction. 1. PCR plate material Its own material is mainly polypropylene (PP) nowadays, which can better adapt
About 96-well plates for PCR experiments, you will understand in three minutes
The 96-well plate used for PCR experiments needs to pay attention to the problem of biological uniformity when doing the inoculation test. First, inoculate one well to see if the protein concentration is appropriate. If appropriate, connect the following well. However, it should be noted that every time a few holes are connected, the culture bottle should be properly shaken to make it evenly distributed as much as possible before taking it. Don't start from the beginning to the end, the culture bottle will sink if it doesn't move.
What are the uses of QPCR transparent heat sealing film?
Heat-sealing film is to seal the film to the container mouth by heating to keep the product fresh, prevent leakage and volatilization, and ensure the safety of product transportation. QPCR transparent heat-sealing film is a polymer transparent film, its advantages are: 1. It can be easily removed, with good optical perme
What should I do if the pipette fails? You will be done after reading this
Tips of several common liquid withdrawal failures: 1. There is residual liquid in the pipette nozzle: This situation occurs because the suction nozzle is not suitable, and the original suction nozzle of the pipette should be replaced immediately. 2. The pipette is blocked and the suction volume is too small: This happens because the liquid has penetr
About nucleic acid detection reagents, those things that have to be said
The detection principle of nucleic acid detection reagents is based on the unique gene sequence of the virus as the detection target. Through PCR amplification, the target DNA sequence of our choice is increased exponentially. Each amplified DNA sequence can be added to us in advance. A piece of fluorescent-labeled probe is combined to generate a fluorescent signal. The more target genes amplified, the stronger the accumulated fluorescent signal. Therefore, nucleic acid detection is actually to determine whether there is viral nucleic acid in the sample by detecti
How to manage consumables in customs laboratories?
Although laboratory consumables are not a theme, the laboratory consumes a large amount of reagent consumables every day. Its importance is self-evident. Strengthening the management of consumables is an important measure to do a good job in laboratory management and ensure the quality of testing work. This article will introduce the scientific and efficient management of laboratory consumables. 1. The importance of laboratory consumables management
●Designate the area where the PCR system is prepared to minimize the entry and exit of items. ●Wear gloves when working in this area and change them frequently. Wear a mask and headgear to reduce the pollution of facial skin and hair cell sources. ●Establish a set of reagents and disposable supplies (including PCR tubes, mineral oil, wax beads, etc.) for individual use. Use new glassware, plastic utensils, and pipettes that have not been exposed to the DNA
Some precautions you have to know about laboratory and reagent consumables management!
Because the laboratory is special and important, its safety affects the hearts of many people. But in terms of laboratory safety, whether it can be managed well depends on many aspects. For example, did the relevant laboratory staff conduct tests in strict accordance with the rules and regulations, did they pay enough attention to them in consciousness; did they complete the relevant registration, and did they follow the standardized operating procedures before and after the test. In addition, there are many laboratory safety issues, including electrical safety, c
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Yongyue Medical tells you the tips for using pipette tips
1. Purpose: to ensure the accuracy of pipetting and the correct use of pipettes. Ensure that the pipette feeds accurately. 2. Scope of application: Adjustable pipettes are used in this laboratory. 3. Purpose of pipette: 3.1
Yongyue Medical: Breaking the monopoly of consumables to be a high-quality national brand
Biological testing is inseparable from equipment, consumables, and reagents. It is the carrier between equipment and reagents, and acts as a "measuring tool" in the experiment. The laboratory is an important factor that affects the success of the experiment. In the experiment, after the reagent and the specimen react, the machine needs to measure the value of the specimen, and the machine has a fixed capacity. If the consumables are not accurate enough, the deviation of the detected experimental data will be Will greatly increase.
(single channel / multi-channel / adjustable / fixed) pipette using the book - tips you don't know
(Single/Multichannel/Adjustable/Fixed) pipettes, no matter which pipette is one of the routine instruments used in our laboratory. The pipetting accuracy and accuracy of the pipette have a great impact on our experimental results. Today, Yongyue medical is sharing some tips that we don't know when using these (single/multi-channel/adjustable/fixed) pipettes. First of all, there are
Pipetting users often have a number of considerations when purchasing pipettes, such as precision, feel, brand, and tip fit. However, when selecting a tip, the user's attention is far less than when the pipette is purchased. There is no shortage of flowers to buy imported pipettes, but it is cheaper to buy consumables. In fact, the quality of the tip will have
What causes the inconsistency of multi-channel pipette tips?
Pipettes are often used in laboratories, schools, research institutes, hospitals, etc. Among the many pipette types, multi-channel pipettes are pipettes with multiple tips, so some users are in use. It is found that the pipette tip suction amount is inconsistent. What causes the multi-channel pipette tip suction amount to be inconsistent? How to solve it? Yongyue Medical please go to the technical staff to answer your questions:
The Global Times reporter learned from Sinopharm Sino Biotech on May 26 that on May 26, the international medical journal "Journal of the American Medical Association" (JAMA) published the "Two Novel Coronavirus Inactivated Vaccines" published by Sinopharm Sino Biotech. Evaluation o
What is the reason why the pipette tip cannot be removed?
Pipettes are used for the pipetting of small or trace amounts of liquid in the laboratory. The specifications of pipettes are different, and pipettes of different specifications are matched with different sizes of pipette tips. In this article, I will share with you why the pipette tip cannot be removed? Customer site: The pipette tip cannot be removed, and the filter tip is broken as soon as it is scraped
Recently, Feng Yan's team from the School of Life Science and Technology of Shanghai Jiaotong University and the State Key Laboratory of Microbial Metabolism published an article entitled "Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations" in Nucle
Which material is good for laboratory consumables
Commonly used pipette tips, pipettes, PCR tube plates, etc. in general laboratories are made of polypropylene, and cell culture plates/cell culture flasks, enzyme-labeled plates or chemiluminescent plates are made of polystyrene, and centrifugation There may be many kinds of tube materials. Take the centrifuge tube as an example to see what are the characteristics of these different plastic materials. Plastic centrifuge tubes are generally made of PP (polypropylene), PC (polycarbonate), PE (polyethylene) and other mater
Uncover the mystery of the laboratory! Into the scientific research laboratory (pipette gun)
In the eyes of most people, scientific research laboratories are full of mystery and high-end atmosphere. In the next period of time, Dian Zijun will take you into the laboratory to learn about the various instruments, consumables and reagents inside, and unveil her mystery. First of all, of course, first introduce the most simple and commonly used instrument-pipette gun. Pipette gun, also known as pipette, is an instrument commonly used in laboratories for quantitatively pipetting small amounts of liquid.
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Privacy statement: Your privacy is very important to Us. Our company promises not to disclose your personal information to any external company with out your explicit permission.